Abstract The flavonoids and xanthones present in the ethanol extracts of leaves and stems of Fridericia samydoides showed that anti-dengue activities in vitro were investigated qualitatively by liquid chromatography-ultraviolet-mass spectrometry in series. Nineteen flavones and fifteen xanthones were detected and characterized on the basis of their fragmentation pattern in the positive and negative ion mode tandem mass spectrometry spectra and ultraviolet bands. Acacetin, chrysin, vitexin, isovitexin, orientin, isoorientin, mangiferin, 2’-O-trans-caffeoylmangiferin, 2’-O-trans-coumaroylmangiferin and 2’-O-trans-cinnamoylmangiferin were identified by comparison with authentic samples. The other compounds detected were tentatively assigned by analysis of the spectral data and by comparison with literature reports. In addition, it performed the fractionation of the leaves extract leading to the isolation of mangiferin, isovitexin and isoorientin. All extracts and isolated compounds inhibited the Dengue virus replication cycle with EC50 less than 25.0 µg/mL for extracts and 272.5, 85.6 and 79.3 µg/mL for mangiferin, isovitexin and isoorientin, respectively.

NANOTECHNOLOGY IN BRAZIL AND THE DEVELOPMENT OF PRODUCTS WITH ANTIMICROBIAL ACTIVITY. Due to the tremendous technological potential and applicability in various industry branches, nanotechnology investments have been increasing over the years. In particular, the control of microorganisms using nanomaterials with antimicrobial action has aroused great interest in the industry. This control makes it necessary in the most diverse situations, such as food conservation, pharmaceutical industry, hospital environments, and even domestic and commercial environments. This extraordinary interest is due, in particular, to the physical-chemical characteristics of materials on a nanoscale that attributes greater efficiency in combating microorganisms. Therefore, researchers and entrepreneurs have directed investments and efforts towards developing new products and/or new applications for antimicrobial products already existing on the market. In this work, we made a brief presentation of the history of nanotechnology in Brazil with a focus on Brazilian companies that developed products or services with nanomaterials with antimicrobial action, and on the description of some of the existing applications in the Brazilian market, highlighting the productive potential of the parents.

A gold nanoparticles functionalization experiment was conducted in a biotechnology summer course at UFOP as a model for the introduction of the laboratory practice in nanotechnology for postgraduate courses in the areas of chemistry and biotechnology. The gold nanorods were synthesized by the seed method and then, functionalized with anti-IL-6 antibodies using the reagents EDAC/NHS and polyethyleneimine (PEI). This nanocompound was tested against the binding with the specific antigen (IL-6) and changes in the longitudinal plasmon absorption spectrum showed the coupling efficiency, which was also verified by the decrease in zeta potential. The experiment was satisfactory, with a positive feedback from participants, and could be implemented in nanotechnology practical classes from postgraduate courses, as a way for improve education in the emergent area of nanobiotechnology.